The present research work describes two simple, sensitive and economically new methods for simultaneous determination of atorvastatin calcium and fenofibrate. The first method was based on UV spectrophotometric determination of two drugs using simultaneous equation method. It involves, the bulk and marketed formulations were estimated using methanol:water (50:50 v/v) as a solvent and maximum absorbance were determined at 243 nm for atorvastatin calcium and 292 nm for fenofibrate. Both the drugs were linear in the concentration range of 5-25 µg/ml and 3-15 µg/ml for atorvastatin calcium and fenofibrate respectively. The correlation coefficients also calculated from the calibration curve for both the drugs respectively were 0.999 and 0.9993 and the method was precise with % RSD of less than 2. The percent recovery was found to be 98.48-99.9 for atorvastatin calcium and 98.7-99.45 for fenofibrate. The second method was based on HPLC separation of two drugs in reverse phase mode using C18 column (Agilent ODS UG 5 Column 250 mm × 4.5 mm). Optimum results were obtained at 273 nm using acetonitrile : methanol : water (50:40:10) v/v as a mobile phase. The chromatograms confirm the presence of atorvastatin calcium and fenofibrate without any interference at 1.5 and 5.11 min, respectively. Linearity obtained in the concentration range of 10-50 µg/ml for both the drugs. The proposed methods were successfully applied for the simultaneous determination of drugs in a pharmaceutical formulation. The developed methods were validated according to ICH guidelines.
Loading....